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Our protocol also leaves the mosquito body intact facilitating other experimental analysis to be undertaken on the same sample. This approach is straightforward and sensitive and allows both species determination and genotyping of Anopheles mosquitoes to be performed in a high-throughput manner. We provide a proof of concept that WGA reactions can be performed directly on mosquito legs, thereby eliminating the need to extract nucleic acid. 89%, respectively), although this difference was not significant. Using three legs instead of one improved genotyping success rates (96% vs.

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We performed nucleic acid extraction on 36 mosquito carcasses and compared the genotype call concordance with their corresponding legs and observed full concordance. We used the Agena Biosciences MassARRAY ® platform (formerly Sequenom) to genotype 78 SNPs for 265 WGA leg samples. Here, we examined the feasibility of using intact portions of a mosquito's leg as sources of template DNA for whole-genome amplification (WGA) by primer-extension preamplification. A major bottleneck of this goal is nucleic acid extraction. Agena will operate as a distinct operating segment under Mesa and its results will be reported as part of a newly-created reportable.

Recent developments in genotyping technologies coupled with the growing desire to characterize genome variation in Anopheles populations open the opportunity to develop more effective genotyping strategies for high-throughput screening.

Agena biosciences